Case Study Levothyroxine Sodium Tablets (25 µg)
Transfer of a manual sample preparation into a fully automated accroma® workflow
The purpose of this study was to compare the manual method of preparation of
Levothyroxine Sodium Tablets (25 µg) for uniformity of content test with a newly
developed robotic preparation using the accroma® robot.
The key point of the method transfer was the extraction time. For the manual preparation 30 minutes sonication with intermediate shaking every 5 minutes were brought down to 3 minutes of extraction time with the accroma®. Not only was full extraction achieved in 3 minutes by the accroma® robot but in addition a slightly greater recovery was achieved when compared to the manual method.
When considering the overall time of preparing a single sample from weighing to transferring the HPLC vial into the liquid chromatograph ready for chromatographic analysis a significant reduction in sample preparation time was observed when preparing sample by the accroma® robot 14 mins compared to 45 minutes by manual means.
- Time saving 68.5% 68.5%
compared to manuel sample preparation